Turk Kardiyol Dern Ars. 2014; 42(1): 55-60 | DOI: 10.5543/tkda.2014.88269
Association between endothelial nitric oxide synthase intron 4a/b polymorphism and aortic dissection
Ahmet Ekmekçi1, Mahmut Uluganyan2, Barış Güngör1, Neslihan Abacı4, Kazım Serhan Özcan1, Gokhan Ertaş1, Aycan Zencirci1, Ahmet Yavuz Balcı3, Sema Sırma Ekmekçi4, Nurten Sayar1, Duran Ustek4, Mehmet Eren11Department of Cardiology, Siyami Ersek Thoracic and Cardiovascular Surgery Center, Training and Research Hospital, Istanbul
2Department of Cardiology, Kadirli State Hospital, Osmaniye
3Department of Cardiovasculary Surgery, Dr. Siyami Ersek Thoracic and Cardiovascular Surgery Center, Training and Research Hospital, İstanbul
4Department of Genetics, Istanbul University, Institute for Experimental Medical Research, Istanbul
OBJECTIVES The genetic risk factors that contribute to the risk of developing aortic dissection (AD) have been studied. We assessed the association of endothelial nitric oxide synthase (eNOS) gene polymorphism with AD.
STUDY DESIGN Patients who underwent surgery with the diagnosis of AD and survived after the operation in our center between May 2007 and June 2011 were recruited retrospectively. The eNOS intron 4a/b polymorphism was determined by polymerase chain reaction (PCR) using oligonucleotide primers (sense: 5’-AGGCCCTATGGTAGTGCCTTT-3’; antisense: 5’-TCTCTTAGTGCTGTGGTCAC-3’) that flank the region of the 27 bp VNTR in intron 4.
RESULTS Thirty-nine patients (88%) had type A AD, while the remainder (12%) had type B AD. The distribution of eNOS4 a/b gene polymorphism differed significantly from the control group, with higher frequencies of eNOS 4a/a and 4a/b genotypes in the AD group (χ2=7.16, p=0.03).
CONCLUSION In this study, the distribution of eNOS genotypes differed between the AD and control groups; however, this polymorphism was not found to be an independent factor for the development of AD.
Keywords: Aortic dissection, eNOS enzyme, genetic predisposition to disease; genotype; introns/genetics; nitric oxide; polymorphism, genetic.
How to cite this article
Ahmet Ekmekçi, Mahmut Uluganyan, Barış Güngör, Neslihan Abacı, Kazım Serhan Özcan, Gokhan Ertaş, Aycan Zencirci, Ahmet Yavuz Balcı, Sema Sırma Ekmekçi, Nurten Sayar, Duran Ustek, Mehmet Eren. Association between endothelial nitric oxide synthase intron 4a/b polymorphism and aortic dissection. Turk Kardiyol Dern Ars. 2014; 42(1): 55-60
Corresponding Author: Ahmet Ekmekçi, Türkiye
Manuscript Language: English